KRT20 Primary Antibody

Item Information
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Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Product Overview
Entrez GenelD
54474
Aliases
K20; CD20; CK20; CK-20; KRT21
Clone#
2C7B9
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human KRT20 (AA: 1-150) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.Genet Mol Res. 2015 May 29;14(2):5823-9. 2.Anticancer Res. 2014 Jan;34(1):159-67.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KRT20 mAb against human KRT20 (AA: 1-150) recombinant protein. (Expected MW is 42.8 kDa)
Figure 2:Western blot analysis using KRT20 mAb against human KRT20 (AA: 1-150) recombinant protein. (Expected MW is 42.8 kDa)
Western Blot
Figure 3:Western blot analysis using KRT20 mAb against HEK293 (1) and KRT20 (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using KRT20 mAb against HEK293 (1) and KRT20 (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using KRT20 mouse mAb with DAB staining.
Figure 4:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using KRT20 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.