Mouse Monoclonal Antibody to KRT2
Item Information
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Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Product Overview
Entrez GenelD
3849
Aliases
K2e; KRTE; CK-2e; KRT2A; KRT2E
Clone#
1A2B5
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human KRT2 (AA: 177-490) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Dermatol Sci. 2016 Jan;81(1):10-6. 2.Acta Histochem. 2016 Jun;118(5):505-12.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KRT2 mAb against human KRT2 (AA: 177-490) recombinant protein. (Expected MW is 52.4 kDa)
Western Blot
Figure 3:Western blot analysis using KRT2 mAb against HEK293-6e (1) and KRT2 (AA: 177-490)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KRT2 mouse mAb against A431 (1) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using KRT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Hela cells using KRT2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using KRT2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using KRT2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.