KRT19 Primary Antibody
Item Information
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Price
Description
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.
Product Overview
Entrez GenelD
3880
Aliases
K19; CK19; K1CS
Clone#
8H1A8
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human KRT19 (AA: 274-400) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.Clin Cancer Res. 2015 Jul 1;21(13):3081-91.
2.Gut. 2014 Apr;63(4):674-85.
2.Gut. 2014 Apr;63(4):674-85.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KRT19 mAb against human KRT19 (AA: 274-400) recombinant protein. (Expected MW is 40 kDa)
Western Blot
Figure 3:Western blot analysis using KRT19 mAb against HEK293 (1) and KRT19 (AA: 274-400)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KRT19 mouse mAb against MCF-7 (1), SW480 (2), SW620 (3), and HCT116 (4) cell lysate.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using KRT19 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using KRT19 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.