KRT19 Primary Antibody

Item Information

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Price

Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Product Overview

Entrez GenelD

3880

Aliases

K19; CK19; K1CS

Clone#

2A1G9

Host / Isotype

Mouse / Mouse IgG1

Immunogen

Purified recombinant fragment of human KRT19 (AA: 80-400) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4?; -20? for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Int J Mol Sci. 2018 May 9;19(5). 2.PLoS One. 2017 Nov 14;12(11):e0187588.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using KRT19 mAb against human KRT19 (AA: 80-400) recombinant protein. (Expected MW is 62.5 kDa)

Western Blot

Figure 3:Western blot analysis using KRT19 mAb against HEK293 (1) and KRT19 (AA: 80-400)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using KRT19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3), SW620 (4), HT-29 (5), HCT116 (6), PC-12 (7), and C6 (8) cell lysate.

Flow Cytometric