This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
Purified antibody in PBS with 0.05% sodium azide
4℃; -20℃ for long term storage
Figure 1:Western blot analysis using KRT14 mAb against human KRT14 (AA: 115-472) recombinant protein. (Expected MW is 43.9 kDa)
Figure 2:Western blot analysis using KRT14 mAb against HEK293-6e (1) and KRT14 (AA: 115-472)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using KRT14 mouse mAb against MCF-7 (1), Hela (2), A431 (3), SW480 (4), T47D (5) and Molt-4 (6) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using KRT14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Flow cytometric analysis of Hela cells using KRT14 mouse mAb (green) and negative control (red).
Figure 6:Immunohistochemical analysis of paraffin-embedded prostatic cancer tissues using KRT14 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded esophagus tissues using KRT14 mouse mAb with DAB staining.
Figure 8:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
For Research Use Only. Not for use in diagnostic procedures.