KRT14

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

Product Overview

Entrez GenelD

3861

Aliases

K14; NFJ; CK14; EBS3; EBS4

Clone#

5D4H4

Host / Isotype

Mouse / Mouse IgG1

Species Reactivity

Human, Monkey

Immunogen

Purified recombinant fragment of human KRT14 (AA: 115-472) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Br J Dermatol. 2020 Oct;183(4):756-757.
2.Br J Dermatol. 2019 Oct;181(4):864-866.

Product Image

Western Blot

Figure 1:Western blot analysis using KRT14 mAb against human KRT14 (AA: 115-472) recombinant protein. (Expected MW is 43.9 kDa)

Western Blot

Figure 2:Western blot analysis using KRT14 mAb against HEK293-6e (1) and KRT14 (AA: 115-472)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 3:Western blot analysis using KRT14 mouse mAb against MCF-7 (1), Hela (2), A431 (3), SW480 (4), T47D (5) and Molt-4 (6) cell lysate.

Immunohistochemical analysis

Figure 4:Immunofluorescence analysis of Hela cells using KRT14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis