Mouse Monoclonal Antibody to KRT13

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Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described.

Product Overview
Entrez GenelD
3860
Aliases
K13; CK13; WSN2
Clone#
7D7A2
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human KRT13 (AA: 104-458) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncotarget. 2016 Dec 20;7(51):84645-84657. 2.BMC Cancer. 2014 Dec 20;14:988.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KRT13 mAb against human KRT13 (AA: 104-458) recombinant protein. (Expected MW is 66.5 kDa)
Figure 2:Western blot analysis using KRT13 mAb against human KRT13 (AA: 104-458) recombinant protein. (Expected MW is 66.5 kDa)
Western Blot
Figure 3:Western blot analysis using KRT13 mAb against HEK293-6e (1) and KRT13 (AA: 104-458)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using KRT13 mAb against HEK293-6e (1) and KRT13 (AA: 104-458)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KRT13 mouse mAb against A431 (1) cell lysate.
Figure 4:Western blot analysis using KRT13 mouse mAb against A431 (1) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of A431 cells using KRT13 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of A431 cells using KRT13 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using KRT13 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using KRT13 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using KRT13 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using KRT13 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KRT13 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KRT13 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.