Mouse Monoclonal Antibody to KRT10

Item Information
Catalog #
Size
Price
Description

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.

Product Overview
Entrez GenelD
3858
Aliases
BIE; EHK; K10; KPP; BCIE; CK10
Clone#
1E4G3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human KRT10 (AA: 146-455) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Cell Mol Med. 2019 Dec;23(12):8442-8452.2.J Eur Acad Dermatol Venereol. 2016 Oct;30(10):e102-e104.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KRT10 mAb against human KRT10 (AA: 146-455) recombinant protein. (Expected MW is 49.1 kDa)
Figure 2:Western blot analysis using KRT10 mAb against human KRT10 (AA: 146-455) recombinant protein. (Expected MW is 49.1 kDa)
Western Blot
Figure 3:Western blot analysis using KRT10 mAb against HEK293-6e (1) and KRT10 (AA: 146-455)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using KRT10 mAb against HEK293-6e (1) and KRT10 (AA: 146-455)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KRT10 mouse mAb against A431 (1), PC-12 (2), and C6 (3) cell lysate.
Figure 4:Western blot analysis using KRT10 mouse mAb against A431 (1), PC-12 (2), and C6 (3) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.