KPNA2 Primary Antibody
Item Information
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Size
Price
Description
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.
Product Overview
Entrez GenelD
3838
Aliases
QIP2; RCH1; IPOA1; SRP1alpha
Clone#
1E8B7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human KPNA2 (AA: 1-530) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mod Pathol. 2014 Jan;27(1):96-106.
2.PLoS One. 2013;8(3):e57911.
2.PLoS One. 2013;8(3):e57911.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KPNA2 mAb against human KPNA2 (AA: 1-530) recombinant protein. (Expected MW is 60.3 kDa)
Western Blot
Figure 3:Western blot analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KPNA2 mouse mAb against Hela (1), HEK293 (2), and NIH/3T3 (3) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using KPNA2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KPNA2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.