KMT5A Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation.
Product Overview
Entrez GenelD
387893
Aliases
SET8; SET07; SETD8; PR-Set7
Clone#
7H1G10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human KMT5A (AA: 109-272) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncotarget. 2016 Jun 7;7(23):34277-87.
2.Cell Rep. 2017 Feb 28;18(9):2148-2161.
2.Cell Rep. 2017 Feb 28;18(9):2148-2161.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KMT5A mAb against human KMT5A (AA: 109-272) recombinant protein. (Expected MW is 44.3 kDa)
Western Blot
Figure 3:Western blot analysis using KMT5A mAb against HEK293 (1) and KMT5A (AA: 109-272)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using KMT5A mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using KMT5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.