KMT5A Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation.

Product Overview

Entrez GenelD

387893

Aliases

SET8; SET07; SETD8; PR-Set7

Clone#

7H1G10

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human KMT5A (AA: 109-272) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/500

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Oncotarget. 2016 Jun 7;7(23):34277-87.
2.Cell Rep. 2017 Feb 28;18(9):2148-2161.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using KMT5A mAb against human KMT5A (AA: 109-272) recombinant protein. (Expected MW is 44.3 kDa)

Western Blot

Figure 3:Western blot analysis using KMT5A mAb against HEK293 (1) and KMT5A (AA: 109-272)-hIgGFc transfected HEK293 (2) cell lysate.

Flow cytometric

Figure 4:Flow cytometric analysis of HL-60 cells using KMT5A mouse mAb (green) and negative control (red).

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using KMT5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.