Mouse Monoclonal Antibody to KMT2A

Item Information

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Price

Description

This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.

Product Overview

Entrez GenelD

4297

Aliases

HRX; MLL; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS

Clone#

7E1D10

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human KMT2A (AA:150-400) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Proc Natl Acad Sci U S A. 2020 Oct 20;117(42):26340-26346.2,Nat Commun. 2020 Dec 21;11(1):6422.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using KMT2A mAb against human KMT2A (AA:150-400) recombinant protein. (Expected MW is 30.9kDa)

Western Blot

Figure 3:Western blot analysis using KMT2A mAb against HEK293-6e (1) and human KMT2A (AA:150-400)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis

Figure 4:Flow cytometric analysis of Hela cells using KMT2A mouse mAb (green) and negative control (red).

Flow cytometric analysis

Figure 5:Flow cytometric analysis of Raji cells using KMT2A mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using KMT2A mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using KMT2A mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using KMT2A mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using KMT2A mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 10:Immunofluorescence analysis of Hela cells using KMT2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.