KMT2A Primary Antibody

Item Information
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Description

This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010]

Product Overview
Entrez GenelD
4297
Aliases
HRX; MLL; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS
Clone#
4A5A12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human KMT2A (AA: 801-956) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.Pediatr Blood Cancer. 2018 Feb;65(2).
2.Pediatr Blood Cancer. 2017 Mar;64(3).
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KMT2A mAb against human KMT2A (AA: 801-956) recombinant protein. (Expected MW is 43.1 kDa)
Figure 2:Western blot analysis using KMT2A mAb against human KMT2A (AA: 801-956) recombinant protein. (Expected MW is 43.1 kDa)
Western Blot
Figure 3:Western blot analysis using KMT2A mAb against HEK293 (1) and KMT2A (AA: 801-956)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using KMT2A mAb against HEK293 (1) and KMT2A (AA: 801-956)-hIgGFc transfected HEK293 (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.