KLHL13 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesBKLHD2; FLJ10262; MGC74791; KLHL13
ProductOrderK
Clone#8D1
Entrez GenelD90293
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human KLHL13 expressed in E. Coli.
MW74kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
325-655
Catalog#: 30067
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Images
Western Blot
Figure 1: Western blot analysis using KLHL13 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.
Figure 1: Western blot analysis using KLHL13 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NTERA-2 cells (left) and U251 (right) cells using KLHL13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of NTERA-2 cells (left) and U251 (right) cells using KLHL13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and pancreas tissues (right) using KLHL13 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and pancreas tissues (right) using KLHL13 mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of 3T3/L1 cells using KLHL13 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of 3T3/L1 cells using KLHL13 mouse mAb (green) and negative control (purple).
Product Overview
Description

KLHL13 (kelch-like 13), also known as BKLHD2 (BTB and kelch domain-containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate- specific adapter of an E3 ubiquitin-protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, KLHL13 interacts with KLHL9 and CUL-3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin-mediated proteolysis. Via its BTB and C-terminal Kelch (BACK) motif, KLHL13 is thought to play a role in spatially orientating substrates in the CUL-3 ligase.

References (references)
References (references)1. DNA Res. 2000 Feb 28;7(1):65-73.
2. Genome Res. 2004 Sep;14(9):1711-8.
3. Cell. 2009 Jul 23;138(2):389-403.