KLHL13 Primary Antibody

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Description

KLHL13 (kelch-like 13), also known as BKLHD2 (BTB and kelch domain-containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate- specific adapter of an E3 ubiquitin-protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, KLHL13 interacts with KLHL9 and CUL-3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin-mediated proteolysis. Via its BTB and C-terminal Kelch (BACK) motif, KLHL13 is thought to play a role in spatially orientating substrates in the CUL-3 ligase.

Product Overview

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

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Western Blot

Figure 1: Western blot analysis using KLHL13 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of NTERA-2 cells (left) and U251 (right) cells using KLHL13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and pancreas tissues (right) using KLHL13 mouse mAb with DAB staining.

Flow cytometric