KLF1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype.
Product Overview
Entrez GenelD
10661
Aliases
EKLF; INLU; HBFQTL6
Clone#
1B6A3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human KLF1 (AA: 208-362) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Biol Chem. 2011 Jul 15;286(28):24819-27.
2. Nat Genet. 2010 Sep;42(9):742-4.
2. Nat Genet. 2010 Sep;42(9):742-4.
Product Image
Western Blot
Figure 1: Western blot analysis using KLF1 mAb against human KLF1 recombinant protein. (Expected MW is 42.6 kDa)
Flow cytometric
Figure 2: Flow cytometric analysis of HeLa cells using KLF1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using KLF1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using KLF1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.