Mouse Monoclonal Antibody to KITLG
Item Information
Catalog #
Size
Price
Description
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
4254
Aliases
SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69
Clone#
2E1F9G6
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human KITLG (AA:Extra(26-214)) expressed in Mammalian.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Stem Cell Res Ther. 2020 Jul 31;11(1):329. 2,Thorac Cancer. 2020 Jul;11(7):1944-1954.
Product Image
Flow cytometric analysis
![Figure 2:Flow cytometric analysis of Hela cells using KITLG mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/099c21ae07c460f4fa5b56f6648f5d662a35f5a280c9f873b4b6f04dacdb7720.jpg?v=1666706031)
Figure 2:Flow cytometric analysis of Hela cells using KITLG mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 3:Flow cytometric analysis of Jurkat cells using KITLG mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1883a05dd22aa69ac9770e99d7a81a91bc40d47411bdbdca5f363362ec7f97aa.jpg?v=1666706031)
Figure 3:Flow cytometric analysis of Jurkat cells using KITLG mouse mAb (green) and negative control (red).
Elisa
![Figure 4:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/6d9d20ba8ec4add76e381c65a94fd453bb4f0fa4d43e48f7c474671ad332ad31.jpg?v=1666706031)
Figure 4:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Raji cells using KITLG mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a167fc118aae7a61e43feacd0c241001455f15100d374a00ee9d49126edb4caf.jpg?v=1666706031)
Figure 5:Flow cytometric analysis of Raji cells using KITLG mouse mAb (green) and negative control (red).
Immunofluorescence analysis
![Figure 6:Immunofluorescence analysis of Hela cells using KITLG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/41affe1b5e9fd9f32aca6d7a1d96caebfa217a92d971eb4c09cbdecb562f531c.jpg?v=1666706031)
Figure 6:Immunofluorescence analysis of Hela cells using KITLG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Western Blot
![Figure 7:Western blot analysis using KITLG mAb against human KITLG (AA: Extra(26-214)) recombinant protein. (Expected MW is 52 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f0bcc725cfc06e9bf0635e28f1bcfb8788561186379481a933fe180422ad88d4.jpg?v=1666706031)
Figure 7:Western blot analysis using KITLG mAb against human KITLG (AA: Extra(26-214)) recombinant protein. (Expected MW is 52 kDa)
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded Bladder cancer tissues using KITLG mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/46a6c6fb55757d6312b5c6c7e9f7f6d4d82df5fa5d47641fbe7f7f6e00e892bf.jpg?v=1666706031)
Figure 7:Immunohistochemical analysis of paraffin-embedded Bladder cancer tissues using KITLG mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.