Mouse Monoclonal Antibody to KITLG
Item Information
Catalog #
Size
Price
Description
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
4254
Aliases
SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69
Clone#
2E1F9G6
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human KITLG (AA:Extra(26-214)) expressed in Mammalian.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Stem Cell Res Ther. 2020 Jul 31;11(1):329. 2,Thorac Cancer. 2020 Jul;11(7):1944-1954.
Product Image
Flow cytometric analysis
Figure 2:Flow cytometric analysis of Hela cells using KITLG mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 3:Flow cytometric analysis of Jurkat cells using KITLG mouse mAb (green) and negative control (red).
Elisa
Figure 4:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Raji cells using KITLG mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using KITLG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Western Blot
Figure 7:Western blot analysis using KITLG mAb against human KITLG (AA: Extra(26-214)) recombinant protein. (Expected MW is 52 kDa)
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded Bladder cancer tissues using KITLG mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.