Mouse Monoclonal Antibody to KIT

Item Information
Catalog #
Size
Price
Description

This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Overview
Entrez GenelD
3815
Aliases
PBT; SCFR; C-Kit; CD117; MASTC
Clone#
1H1B1
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human KIT (AA: 23-322) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Genes Chromosomes Cancer. 2019 Nov;58(11):798-803. 2.Exp Hematol. 2019 Jun;74:19-24.e4.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KIT mAb against human KIT (AA: 23-322) recombinant protein. (Expected MW is 59.6 kDa)
Figure 2:Western blot analysis using KIT mAb against human KIT (AA: 23-322) recombinant protein. (Expected MW is 59.6 kDa)
Western Blot
Figure 3:Western blot analysis using KIT mAb against HEK293-6e (1) and KIT (AA: 23-322)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using KIT mAb against HEK293-6e (1) and KIT (AA: 23-322)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using KIT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using KIT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of K562 cells using KIT mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of K562 cells using KIT mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.