KIR3DL1 Primary Antibody

Item Information
Catalog #
Size
Price
Description
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response.
Product Overview
Entrez GenelD
3811
Aliases
KIR; NKB1; NKAT3; NKB1B; NKAT-3; CD158E1; KIR3DL1/S1
Clone#
6D9A4
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human KIR3DL1 (AA: extra 22-340) expressed in HEK293 cells.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Clin Exp Immunol. 2016 Mar;183(3):419-30.
2.J Leukoc Biol. 2010 Nov;88(5):905-12.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KIR3DL1 mAb against human KIR3DL1 (AA: extra 22-340) recombinant protein. (Expected MW is 65 kDa)
Figure 2:Western blot analysis using KIR3DL1 mAb against human KIR3DL1 (AA: extra 22-340) recombinant protein. (Expected MW is 65 kDa)
Western Blot
Figure 3:Western blot analysis using KIR3DL1 mouse mAb against A431 (1), Raji (2), SPC-A-1 (3), K562 (4), HEK293 (5), U937 (6), and C6 (7) cell lysate.
Figure 3:Western blot analysis using KIR3DL1 mouse mAb against A431 (1), Raji (2), SPC-A-1 (3), K562 (4), HEK293 (5), U937 (6), and C6 (7) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Jurkat cells using KIR3DL1 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Jurkat cells using KIR3DL1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.