Ki67 Primary Antibody

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Description

Ki67: antigen identified by monoclonal antibody Ki-67. Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Product Overview
Entrez GenelD
4288
Aliases
KIA; Ki-67; MKI67
Clone#
9C12B2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of Ki167 (aa3118-3256) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Folia Histochem Cytobiol. 2007;45(4):357-66.
2. Tumori. 2008 May-Jun;94(3):389-97.
Product Image
Western Blot
Figure 1: Western blot analysis using Ki67 mouse mAb against truncated Trx-Ki67 recombinant protein(1),truncated Ki67 (aa3118-3256)-His recombinant protein(2) and truncated Ki67 (aa3118-3256)-hIgGFc transfected CHO-K1 cell lysate(3).
Figure 1: Western blot analysis using Ki67 mouse mAb against truncated Trx-Ki67 recombinant protein(1),truncated Ki67 (aa3118-3256)-His recombinant protein(2) and truncated Ki67 (aa3118-3256)-hIgGFc transfected CHO-K1 cell lysate(3).
For Research Use Only. Not for use in diagnostic procedures.