KDM4A Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesJMJD2; JHDM3A; JMJD2A; TDRD14A
ProductOrderK
Clone#6E10G4
Entrez GenelD9682
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human KDM4A (AA: 932-1057) expressed in E. Coli.
MW120.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
932-1057
Catalog#: 30903
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using KDM4A mAb against human KDM4A (AA: 932-1057) recombinant protein. (Expected MW is 40.6 kDa)
Figure 2:Western blot analysis using KDM4A mAb against human KDM4A (AA: 932-1057) recombinant protein. (Expected MW is 40.6 kDa)
Western Blot
Figure 3:Western blot analysis using KDM4A mAb against HEK293 (1) and KDM4A (AA: 932-1057)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using KDM4A mAb against HEK293 (1) and KDM4A (AA: 932-1057)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of HeLa cells using KDM4A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 4:Immunofluorescence analysis of HeLa cells using KDM4A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of HeLa cells using KDM4A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of HeLa cells using KDM4A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Product Overview
DescriptionThis gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
References (references)
References (references)1.Asian Pac J Cancer Prev. 2014;15(7):3051-6.
2.J Cell Biochem. 2012 Apr;113(4):1368-76.