KDM4A Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
Product Overview
Entrez GenelD
9682
Aliases
JMJD2; JHDM3A; JMJD2A; KIAA0677; KDM4A
Clone#
5H1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human KDM4A expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Genome Res. 2004 Sep;14(9):1711-8.
2. Nat Methods. 2005 Aug;2(8):591-8.
3. Cell. 2006 May 19;125(4):691-702.
2. Nat Methods. 2005 Aug;2(8):591-8.
3. Cell. 2006 May 19;125(4):691-702.
Product Image
Western Blot
Figure 1: Western blot analysis using KDM4A mAb against HEK293 (1) and KDM4A(AA: 500-705)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human larynx cancer tissues (right) using KDM4A mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of NTERA-2 cells using KDM4A mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
For Research Use Only. Not for use in diagnostic procedures.