KDM1A Primary Antibody
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Description
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using KDM1A mAb against human KDM1A (AA: 55-263) recombinant protein. (Expected MW is 60.3 kDa)
Western Blot

Figure 3:Western blot analysis using KDM1A mAb against HEK293 (1) and KDM1A (AA: 55-263)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 4:Western blot analysis using KDM1A mouse mAb against K562 (1), Jurkat (2), and Hela (3) cell lysate.
Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of HL-7702 cells using KDM1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis

Figure 6:Immunofluorescence analysis of MCF-7 cells using KDM1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric

Figure 7:Flow cytometric analysis of Hela cells using KDM1A mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KDM1A mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 9:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using KDM1A mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.