KDM1A Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
23028
Aliases
AOF2; CPRF; KDM1; LSD1; BHC110
Clone#
1H2F8
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human KDM1A (AA: 709-876) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int J Clin Exp Pathol. 2014 Dec 1;7(12):8929-34. 2.Blood. 2014 Jul 3;124(1):151-2.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KDM1A mAb against human KDM1A (AA: 709-876) recombinant protein. (Expected MW is 43.8 kDa)
Western Blot
Figure 3:Western blot analysis using KDM1A mAb against HEK293 (1) and KDM1A (AA: 709-876)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KDM1A mouse mAb against COS7 (1), NIH/3T3 (2), and A549 (3) cell lysate.
Flow Cytometric
Figure 5:Flow cytometric analysis of Hela cells using KDM1A mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using KDM1A mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KDM1A mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.