Mouse Monoclonal Antibody to KCNN4

Item Information
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Description

The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily.

Product Overview
Entrez GenelD
3783
Aliases
IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1
Clone#
1B4B4
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human KCNN4 (AA: extra 286-427) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Exp Cell Res. 2018 Aug 15;369(2):208-217. 2.Sci Signal. 2018 Apr 24;11(527):eaaq1616.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KCNN4 mAb against human KCNN4 (AA: extra 286-427) recombinant protein. (Expected MW is 42.7 kDa)
Figure 2:Western blot analysis using KCNN4 mAb against human KCNN4 (AA: extra 286-427) recombinant protein. (Expected MW is 42.7 kDa)
Western Blot
Figure 3:Western blot analysis using KCNN4 mAb against HEK293-6e (1) and KCNN4 (AA: extra 286-427)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using KCNN4 mAb against HEK293-6e (1) and KCNN4 (AA: extra 286-427)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using KCNN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using KCNN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using KCNN4 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of THP-1 cells using KCNN4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.