Mouse Monoclonal Antibody to KCNN4
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily.
Product Overview
Entrez GenelD
3783
Aliases
IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1
Clone#
1B4B4
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human KCNN4 (AA: extra 286-427) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Exp Cell Res. 2018 Aug 15;369(2):208-217. 2.Sci Signal. 2018 Apr 24;11(527):eaaq1616.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/446a02c128a1f3e6dd72e8fb36f03a3e6bd72a027f675bea5d2bdf41b41c7e53.jpg?v=1666705972)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using KCNN4 mAb against human KCNN4 (AA: extra 286-427) recombinant protein. (Expected MW is 42.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/207f9cf679ef637e4eb217c640446c6bfbf7b9190fe8a23768e824a4923f9575.jpg?v=1666705972)
Figure 2:Western blot analysis using KCNN4 mAb against human KCNN4 (AA: extra 286-427) recombinant protein. (Expected MW is 42.7 kDa)
Western Blot
![Figure 3:Western blot analysis using KCNN4 mAb against HEK293-6e (1) and KCNN4 (AA: extra 286-427)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a031c57551e97632ba60757de0dc5c332bbb2da129aed2a97f6869f5a7273e53.jpg?v=1666705972)
Figure 3:Western blot analysis using KCNN4 mAb against HEK293-6e (1) and KCNN4 (AA: extra 286-427)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
![Figure 4:Immunofluorescence analysis of Hela cells using KCNN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/44b1388173b010d4406dd21a68850ea830f8dce63c851eb235718918b08dd0ee.jpg?v=1666705972)
Figure 4:Immunofluorescence analysis of Hela cells using KCNN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of THP-1 cells using KCNN4 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a84939bd122d02c8a9b0bc529b350ca52e2fb432fb55f5b10d1430fa72e21415.jpg?v=1666705972)
Figure 5:Flow cytometric analysis of THP-1 cells using KCNN4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.