Mouse Monoclonal Antibody to KCNN4
Item Information
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Price
Description
The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily.
Product Overview
Entrez GenelD
3783
Aliases
IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1
Clone#
2B1G1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human KCNN4 (AA: extra 286-427) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Exp Cell Res. 2018 Aug 15;369(2):208-217. 2.Sci Signal. 2018 Apr 24;11(527):eaaq1616.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b1f237919db6ded9bf4ac8c80b05a583e248cccc850cb56de1f70a7b86676896.jpg?v=1666705848)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using KCNN4 mAb against human KCNN4 (AA: extra 286-427) recombinant protein. (Expected MW is 42.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/85937c81d4f81ac4a8144b6e27a5d0f88fc7e894be118bb35736087a8961c7a0.jpg?v=1666705848)
Figure 2:Western blot analysis using KCNN4 mAb against human KCNN4 (AA: extra 286-427) recombinant protein. (Expected MW is 42.7 kDa)
Western Blot
![Figure 3:Western blot analysis using KCNN4 mAb against HEK293-6e (1) and KCNN4 (AA: extra 286-427)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/437ce5c4c741c4afa27616321a1e4df05b354c16662a8e2238fb066e143722c0.jpg?v=1666705848)
Figure 3:Western blot analysis using KCNN4 mAb against HEK293-6e (1) and KCNN4 (AA: extra 286-427)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using KCNN4 mouse mAb against HepG2 (1), Jurkat (2), Hela (3), MCF-7 (4), HEK293 (5), SW480 (6), HT-29 (7), and SW620 (8) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c7b519c633b61dcf554cc9cedf186575d7629ba3caee4608989ca9df20f577e2.jpg?v=1666705848)
Figure 4:Western blot analysis using KCNN4 mouse mAb against HepG2 (1), Jurkat (2), Hela (3), MCF-7 (4), HEK293 (5), SW480 (6), HT-29 (7), and SW620 (8) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of THP-1 cells using KCNN4 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d3f78f85d62059e5587bc63cb8af9f4dbacd95d91cc753c3a31efe95a7c2b05d.jpg?v=1666705848)
Figure 5:Flow cytometric analysis of THP-1 cells using KCNN4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.