Mouse Monoclonal Antibody to KCNK1
Item Information
Catalog #
Size
Price
Description
This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
3775
Aliases
DPK; HOHO; K2P1; KCNO1; TWIK1; K2p1.1; TWIK-1
Clone#
5E10A5
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human KCNK1 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Biophys J. 2016 Aug 23;111(4):775-784. 2,J Biol Chem. 2020 Jan 10;295(2):610-618.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/19ff1ee0da085f1bac04272eb6edebc51c705fa671c8e884bf67adb635bf0c58.jpg?v=1666705341)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using KCNK1 mAb against human KCNK1 (AA: extra mix) recombinant protein. (Expected MW is 36.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7aab5672afdbdb28eaebb72fb033b5853d7c87e4a740fc1740eda9999e11d9a2.jpg?v=1666705341)
Figure 2:Western blot analysis using KCNK1 mAb against human KCNK1 (AA: extra mix) recombinant protein. (Expected MW is 36.7 kDa)
Western Blot
![Figure 3:Western blot analysis using KCNK1 mAb against HEK293-6e (1) and KCNK1 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8d358e10003c9bcfbc82283558d6e789d22f2082b42276c5e54742b9ba9b8d37.jpg?v=1666705341)
Figure 3:Western blot analysis using KCNK1 mAb against HEK293-6e (1) and KCNK1 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of C6 cells using KCNK1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e3b74c44857a853908c31658764a12f6c32fd0844791e91267c5c1266925a04a.jpg?v=1666705341)
Figure 4:Flow cytometric analysis of C6 cells using KCNK1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of HepG2 cells using KCNK1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/363c55121a65ee40c154208d4d5d1d9c54d73851aa6a06508cfde2d7dcb8315e.jpg?v=1666705341)
Figure 5:Flow cytometric analysis of HepG2 cells using KCNK1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of SK-N-SH cells using KCNK1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ea8618048ba0f6dca24c8c6692126a7ebb0d07b1481348730dcf8f406eaab987.jpg?v=1666705341)
Figure 6:Flow cytometric analysis of SK-N-SH cells using KCNK1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.