Mouse Monoclonal Antibody to KCNK1

Item Information
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Price
Description

This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq, Jul 2008]

Product Overview
Entrez GenelD
3775
Aliases
DPK; HOHO; K2P1; KCNO1; TWIK1; K2p1.1; TWIK-1
Clone#
5E10A5
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human KCNK1 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Biophys J. 2016 Aug 23;111(4):775-784. 2,J Biol Chem. 2020 Jan 10;295(2):610-618.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KCNK1 mAb against human KCNK1 (AA: extra mix) recombinant protein. (Expected MW is 36.7 kDa)
Figure 2:Western blot analysis using KCNK1 mAb against human KCNK1 (AA: extra mix) recombinant protein. (Expected MW is 36.7 kDa)
Western Blot
Figure 3:Western blot analysis using KCNK1 mAb against HEK293-6e (1) and KCNK1 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using KCNK1 mAb against HEK293-6e (1) and KCNK1 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of C6 cells using KCNK1 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of C6 cells using KCNK1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using KCNK1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HepG2 cells using KCNK1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of SK-N-SH cells using KCNK1 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of SK-N-SH cells using KCNK1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.