KARS Primary Antibody

Item Information

Catalog #

Size

Price

Description

KARS: lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Product Overview

Entrez GenelD

3735

Aliases

KRS; KARS2; KIAA0070

Clone#

8G12C1

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. J Biol Chem. 2000 Nov 10;275(45):35063-9.
2. J Virol. 2001 Jun;75(11):5043-8.
3. Biochem Biophys Res Commun. 2002 Feb 15;291(1):158-64.
4. Cell. 2004 Oct 15;119(2):195-208.

Product Image

Western Blot

Figure 1: Western blot analysis using KARS mouse mAb against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, showing cytoplasmic localization with DAB staining using KARS mouse mAb.

Immunohistochemical analysis