JAK3 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesJAKL; LJAK
ProductOrderJ
Clone#5H2
Entrez GenelD3718
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human JAK3 expressed in E. Coli.
MW125kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
116-328
Catalog#: 20400
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Images
Western Blot
Figure 1: Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).
Figure 1: Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Confocal Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Confocal Immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and negative control (red).
Product Overview
Description

JAK3, Janus kinase 3. It is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).

References (references)
References (references)1. J Biol Chem. 1995 Oct 20;270(42):25028-36.
2. Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6374-8.
3. Leuk Lymphoma. 2002 Dec;43(12):2355-62.