JAK3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
JAK3, Janus kinase 3. It is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).
Product Overview
Entrez GenelD
3718
Aliases
JAKL; LJAK
Clone#
5H2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human JAK3 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Biol Chem. 1995 Oct 20;270(42):25028-36.
2. Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6374-8.
3. Leuk Lymphoma. 2002 Dec;43(12):2355-62.
2. Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6374-8.
3. Leuk Lymphoma. 2002 Dec;43(12):2355-62.
Product Image
Western Blot
Figure 1: Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.