ITK Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesEMT; LYK; PSCTK2; MGC126257; MGC126258
ProductOrderI
Clone#5G6
Entrez GenelD3702
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ITK expressed in E. Coli.
MW72kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
2-120
Catalog#: 30244
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Images
Western Blot
Figure 1: Western blot analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)
Figure 1: Western blot analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)
Western Blot
Figure 2: Western blot analysis using ITK mouse mAb against Jurkat cell lysate.
Figure 2: Western blot analysis using ITK mouse mAb against Jurkat cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
Figure 4: Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.

References (references)
References (references)1. J Clin Invest. 2009 May;119(5):1350-8.
2. Mol Cells. 2009 Aug 31;28(2):125-30.