ITK Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.
Product Overview
Entrez GenelD
3702
Aliases
EMT; LYK; PSCTK2; MGC126257; MGC126258
Clone#
5G6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ITK expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Clin Invest. 2009 May;119(5):1350-8.
2. Mol Cells. 2009 Aug 31;28(2):125-30.
2. Mol Cells. 2009 Aug 31;28(2):125-30.
Product Image
Western Blot
Figure 1: Western blot analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)
Western Blot
Figure 2: Western blot analysis using ITK mouse mAb against Jurkat cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.