Mouse Monoclonal Antibody to ITGB5

Item Information
Catalog #Size/ConcentrationPrice
Specification
Aliasesundefined
Clone#2B5A7
Entrez GenelD3693
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2a
ImmunogenPurified recombinant fragment of human ITGB5 (AA: 50-319) expressed in E. Coli.
MW88kDa
Application
ELISA1/10000
ICC (Immunocytochemistry)1/50 - 1/200
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
50-319
Catalog#: 32268
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ITGB5 mAb against human ITGB5 (AA: 50-319) recombinant protein. (Expected MW is 33 kDa)
Figure 2:Western blot analysis using ITGB5 mAb against human ITGB5 (AA: 50-319) recombinant protein. (Expected MW is 33 kDa)
Western Blot
Figure 3:Western blot analysis using ITGB5 mAb against HEK293-6e (1) and ITGB5 (AA: 50-319)-hIgGFc transfected ITGB5 (2) cell lysate.
Figure 3:Western blot analysis using ITGB5 mAb against HEK293-6e (1) and ITGB5 (AA: 50-319)-hIgGFc transfected ITGB5 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using ITGB5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using ITGB5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using ITGB5 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Jurkat cells using ITGB5 mouse mAb (green) and negative control (red).
Product Overview
Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

References (references)
References (references)1.Nucleic Acids Res. 2020 Dec 16;48(22):12727-12745. 2.Prog Transplant. 2020 Jun;30(2):184-186.