Mouse Monoclonal Antibody to ITGB5
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Description
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ITGB5 mAb against human ITGB5 (AA: 50-319) recombinant protein. (Expected MW is 33 kDa)
Western Blot
Figure 3:Western blot analysis using ITGB5 mAb against HEK293-6e (1) and ITGB5 (AA: 50-319)-hIgGFc transfected ITGB5 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using ITGB5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using ITGB5 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.