Mouse Monoclonal Antibody to ITGB5
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | undefined |
| Clone# | 2B5A7 |
| Entrez GenelD | 3693 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG2a |
| Immunogen | Purified recombinant fragment of human ITGB5 (AA: 50-319) expressed in E. Coli. |
| MW | 88kDa |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/50 - 1/200 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 50-319 | |
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ITGB5 mAb against human ITGB5 (AA: 50-319) recombinant protein. (Expected MW is 33 kDa)
Western Blot
Figure 3:Western blot analysis using ITGB5 mAb against HEK293-6e (1) and ITGB5 (AA: 50-319)-hIgGFc transfected ITGB5 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using ITGB5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using ITGB5 mouse mAb (green) and negative control (red).