Mouse Monoclonal Antibody to ITGB5
Item Information
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Price
Description
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
3693
Aliases
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Clone#
1A4H1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human ITGB5 (AA: extra 50-319) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Nucleic Acids Res. 2020 Dec 16;48(22):12727-12745. 2.Prog Transplant. 2020 Jun;30(2):184-186.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ITGB5 mAb against human ITGB5 (AA: extra 50-319) recombinant protein. (Expected MW is 33 kDa)
Western Blot
Figure 3:Western blot analysis using ITGB5 mAb against HEK293-6e (1) and ITGB5 (AA: extra 50-319)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Jurkat cells using ITGB5 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.