ITGAM Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
3684
Aliases
CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Clone#
3A10H5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ITGAM (AA: 623-728) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Ann Rheum Dis. 2012 Dec;71(12):2028-34.
2. J Infect Chemother. 2011 Apr;17(2):291-6.
2. J Infect Chemother. 2011 Apr;17(2):291-6.
Product Image
Western Blot
Figure 1: Western blot analysis using ITGAM mAb against human ITGAM recombinant protein. (Expected MW is 37.5 kDa)
Western Blot
Figure 2: Western blot analysis using ITGAM mAb against HEK293 (1) and ITGAM (AA: 623-728)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using ITGAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.