ISL1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Product Overview

Entrez GenelD

3670

Aliases

Isl-1; ISLET1

Clone#

1B1

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human ISL1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Cell. 1996 Jan 26;84(2):309-20.
2. J Neurosci. 2008 Mar 26;28(13):3291-7.

Product Image

Western Blot

Figure 1: Western blot analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).

Immunofluorescence analysis

Figure 3: Confocal Immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

For Research Use Only. Not for use in diagnostic procedures.