ISL1 Primary Antibody

Item Information

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Size

Price

Description

ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Product Overview

Entrez GenelD

3670

Aliases

Isl-1; ISLET1

Clone#

1H9

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human ISL1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Cell. 1996 Jan 26;84(2):309-20.
2. J Neurosci. 2008 Mar 26;28(13):3291-7.

Product Image

Western Blot

Figure 1: Western blot analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and cervical carcinoma (right), showing nuclear localization using ISL1 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 3: Confocal Immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

For Research Use Only. Not for use in diagnostic procedures.