IRF3 Primary Antibody
Item Information
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Size
Price
Description
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.
Product Overview
Entrez GenelD
3661
Aliases
IIAE7
Clone#
2A10A8
Host / Isotype
Mouse / Mouse IgG2b
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human IRF3 (AA: 1-150) expressed in HEK293-6e cells supernatant.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Immunol. 2020 Oct 15;205(8):1981-1989.
2.Viruses. 2019 Mar 12;11(3):246.
2.Viruses. 2019 Mar 12;11(3):246.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using IRF3 mAb against human IRF3 (AA: 1-150) recombinant protein. (Expected MW is 47.4 kDa)
Immunohistochemical analysis
Figure 3:Immunofluorescence analysis of Hela cells using IRF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of NIH/3T3 cells using IRF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of RSC96 cells using IRF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of Hela cells using IRF3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded mouse liver tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 12:Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 13:Immunohistochemical analysis of paraffin-embedded rat liver tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 14:Immunohistochemical analysis of paraffin-embedded rat kidney tissues using IRF3 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.