IRF3
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | IIAE7 |
| Clone# | 2A10A8 |
| Entrez GenelD | 3661 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Immunogen | Purified recombinant fragment of human IRF3 (AA: 1-150) expressed in HEK293-6e cells supernatant. |
| MW | 47.2kDa |
| Species Reactivity | Human, Mouse, Rat |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 1-150 | |
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using IRF3 mAb against human IRF3 (AA: 1-150) recombinant protein. (Expected MW is 47.4 kDa)
Immunohistochemical analysis
Figure 3:Immunofluorescence analysis of Hela cells using IRF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of NIH/3T3 cells using IRF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of RSC96 cells using IRF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of Hela cells using IRF3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded mouse liver tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 12:Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 13:Immunohistochemical analysis of paraffin-embedded rat liver tissues using IRF3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 14:Immunohistochemical analysis of paraffin-embedded rat kidney tissues using IRF3 mouse mAb with DAB staining.