IRAK3 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesASRT5; IRAKM
ProductOrderI
Clone#5C3D6
Entrez GenelD11213
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human IRAK3 (AA: 454-596) expressed in E. Coli.
MW67.8kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/100 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
454-596
Catalog#: 30786
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using IRAK3 mAb against human IRAK3 (AA: 454-596) recombinant protein. (Expected MW is 42.3 kDa)
Figure 2:Western blot analysis using IRAK3 mAb against human IRAK3 (AA: 454-596) recombinant protein. (Expected MW is 42.3 kDa)
Western Blot
Figure 3:Western blot analysis using IRAK3 mAb against HEK293 (1) and IRAK3 (AA: 454-596)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using IRAK3 mAb against HEK293 (1) and IRAK3 (AA: 454-596)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of A549 cells using IRAK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of A549 cells using IRAK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Product Overview
DescriptionThis gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants.
References (references)
References (references)1.PLoS One. 2012;7(1):e30414.
2.Am J Respir Cell Mol Biol. 2011 Oct;45(4):740-5.