IL3RA Primary Antibody

Item Information
Catalog # Size/Concentration Price
30516 100μl $341.00
Specification
AliasesIL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Product OrderI
Clone#8E11C5
Entrez GeneID3563
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human IL3RA (AA: 200-305) expressed in E. Coli.
MW43.3kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P (Immunohistochemistry) 1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
Sequence
200-305
Catalog
30516
$341.00
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Images

Western Blot

Cell Culture Products
Figure 1: Western blot analysis using IL3RA mAb against human IL3RA recombinant protein. (Expected MW is 38.3 kDa)

Western Blot

Cell Culture Products
Figure 2: Western blot analysis using IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Cell Culture Products
Figure 3: Immunofluorescence analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric

Cell Culture Products
Figure 4: Flow cytometric analysis of Hela cells using IL3RA mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Cell Culture Products
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using IL3RA mouse mAb with DAB staining.

Immunohistochemical analysis

Cell Culture Products
Figure 6: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IL3RA mouse mAb with DAB staining.

Elisa

Cell Culture Products
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer -
B1/2 Esophageal Cancer -
C1/2 Esophagus -
D1/2 Esophagus -
E1/2 Stomach Cancer -
F1/2 Stomach Cancer -
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer +
C3/4 Colon -
D3/4 Duodenum -
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver +
D5/6 Renal cell Carcinoma +
E5/6 Kidney -
F5/6 Pancreas -
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer +
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer -
B9/10 Breast Cancer -
C9/10 Ovarian Cancer -
D9/10 Ovarian Cancer +
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer -
H9/10 Myocardial -
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Product Overview
Description The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.



References (references)
References (references) 1. Am J Clin Pathol. 2011 Oct;136(4):625-30.
2. Chin Med J (Engl). 2010 Aug 5;123(15):2034-7.

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