IL3RA Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.
Product Overview
Entrez GenelD
3563
Aliases
IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Clone#
8E11C5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human IL3RA (AA: 200-305) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Am J Clin Pathol. 2011 Oct;136(4):625-30.
2. Chin Med J (Engl). 2010 Aug 5;123(15):2034-7.
2. Chin Med J (Engl). 2010 Aug 5;123(15):2034-7.
Product Image
Western Blot
Figure 1: Western blot analysis using IL3RA mAb against human IL3RA recombinant protein. (Expected MW is 38.3 kDa)
Western Blot
Figure 2: Western blot analysis using IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using IL3RA mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using IL3RA mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IL3RA mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.