IL34 Primary Antibody
Item Information
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Size
Price
Description
Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R.Tissue specificity: Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.
Product Overview
Entrez GenelD
146433
Aliases
IL-34; C16orf77; MGC34647; IL34
Clone#
1D12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human IL34 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24.
2. Lim J, et al. Cell, 2006 May 19.
3. Lin H, et al. Science, 2008 May 9.
2. Lim J, et al. Cell, 2006 May 19.
3. Lin H, et al. Science, 2008 May 9.
Product Image
Western Blot
Figure 1: Western blot analysis using IL34 mAb against IL34-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of U251 cells using IL34 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 3: Flow cytometric analysis of K562 cells using IL34 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.