IL1B Primary Antibody

Item Information

Catalog #

Size

Price

Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Product Overview

Entrez GenelD

3553

Aliases

IL-1; IL1F2; IL1-BETA

Clone#

3A6

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human IL1B expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Cell Signal. 2009 Dec;21(12):1935-44.
2. Nat Struct Mol Biol. 2009 Sep;16(9):945-52.

Product Image

Western Blot

Figure 1: Western blot analysis using IL1B mAb against human IL1B (AA: 126-261) recombinant protein. (Expected MW is 41 kDa)

Western Blot

Figure 2: Western blot analysis using IL1B mAb against HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IL1B mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 5: Immunofluorescence analysis of HepG2 cells using IL1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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