IL10RB Primary Antibody

Item Information
Catalog #
Size
Price
Description

The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.

Product Overview
Entrez GenelD
3588
Aliases
CRFB4; CRF2-4; D21S58; D21S66; CDW210B; IL-10R2
Clone#
5B3F2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human IL10RB (AA: extra 20-220) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Immunol Res. 2015 Nov;3(11):1227-35.
2.Biochem Biophys Res Commun. 2008 Aug 29;373(3):403-7.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using IL10RB mAb against human IL10RB (AA: extra 20-220) recombinant protein. (Expected MW is 49.5 kDa)
Figure 2:Western blot analysis using IL10RB mAb against human IL10RB (AA: extra 20-220) recombinant protein. (Expected MW is 49.5 kDa)
Western Blot
Figure 3:Western blot analysis using IL10RB mAb against HEK293 (1) and IL10RB (AA: extra 20-220)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using IL10RB mAb against HEK293 (1) and IL10RB (AA: extra 20-220)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using IL10RB mouse mAb against Hela (1), Jurkat (2), Ramos (3), Raji (4), and C6 (5) cell lysate.
Figure 4:Western blot analysis using IL10RB mouse mAb against Hela (1), Jurkat (2), Ramos (3), Raji (4), and C6 (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HL-60 cells using IL10RB mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HL-60 cells using IL10RB mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.