Mouse Monoclonal Antibody to IKZF1
This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0a32ec6515908b80d5c353411286220372a0c9e727f3963da484ba1810b395b8.jpg?v=1666705651)
![Figure 2:Western blot analysis using IKZF1 mAb against human IKZF1 (AA: 401-520) recombinant protein. (Expected MW is 31.2kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/02075200773978c68e5aa10ba2900b4a6ac1cd9cf75d2fb22d6e0c8b4b8ce1f9.jpg?v=1666705652)
![Figure 3:Western blot analysis using IKZF1 mAb against HEK293-6e (1) and IKZF1 (AA: 401-520)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/96f82d1aa61257aac9e17cc1be0d05f4f417907c9701fae8f689fbef22c706f3.jpg?v=1666705652)
![Figure 4:Flow cytometric analysis of Jurkat cells using IKZF1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4f3b29b5dde29a2b5c10a05ffae5672f6f149537188ad16bc4af4f434ae72a1e.jpg?v=1666705652)