Mouse Monoclonal Antibody to IKZF1

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Description

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).

Product Overview
Entrez GenelD
10320
Aliases
IK1; LYF1; LyF-1; CVID13; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452
Clone#
4B6A12
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human IKZF1 (AA: 401-520) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Observational Study Medicine (Baltimore). 2020 Oct 9;99(41):e22607.2,J Pediatr Hematol Oncol. 2020 Oct;42(7):420-428.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using IKZF1 mAb against human IKZF1 (AA: 401-520) recombinant protein. (Expected MW is 31.2kDa)
Figure 2:Western blot analysis using IKZF1 mAb against human IKZF1 (AA: 401-520) recombinant protein. (Expected MW is 31.2kDa)
Western Blot
Figure 3:Western blot analysis using IKZF1 mAb against HEK293-6e (1) and IKZF1 (AA: 401-520)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using IKZF1 mAb against HEK293-6e (1) and IKZF1 (AA: 401-520)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Jurkat cells using IKZF1 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Jurkat cells using IKZF1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.