Mouse Monoclonal Antibody to IKZF1
This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d1876c53f3c29fee5e5c92e6f4a88499f8c42fc3dd0b1bdcb1cc886b67e80949.jpg?v=1666705649)
![Figure 2:Western blot analysis using IKZF1 mAb against human IKZF1 (AA: 401-520) recombinant protein. (Expected MW is 31.2kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a54b160587f3fe9bfe33564e2bdb4c132574aecfeac745e313263c398773f9ca.jpg?v=1666705649)
![Figure 3:Western blot analysis using IKZF1 mAb against HEK293-6e (1) and IKZF1 (AA: 401-520)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/081675c9f1b6d2ac8a5b973bc67430f0d1651051af1aeb3792407f66fe07cf6d.jpg?v=1666705649)
![Figure 4:Flow cytometric analysis of Jurkat cells using IKZF1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7a106505484d71427bc09683654a4f80acde02a89eaf78c08a922ffea8b14d6b.jpg?v=1666705649)
![Figure 5:Immunofluorescence analysis of Hela cells using IKZF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d604ec643146e021260d4d8928f03fc758669878dfab076e71c1be0ba8cacc93.jpg?v=1666705649)