IHOG Primary Antibody

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Description

The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.

Product Overview
Entrez GenelD
33972
Aliases
CG9211; CT26314; Dmel CG9211; ihog; Ihog
Clone#
3G8
Host / Isotype
Mouse / IgG1
Species Reactivity
Drosophila Melanogaster
Immunogen
Purified recombinant fragment of human IHOG expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Cell. 2006 Apr 21;125(2):343-57.
2. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17208-13.
Product Image
Western Blot
Figure 1: Western blot analysis using IHOG mAb against IHOG-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using IHOG mAb against IHOG-hIgGFc transfected HEK293 cell lysate.
For Research Use Only. Not for use in diagnostic procedures.