IHOG Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCG9211; CT26314; Dmel CG9211; ihog; Ihog
ProductOrderI
Clone#3G8
Entrez GenelD33972
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human IHOG expressed in E. Coli.
MW98kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityDrosophila Melanogaster
Application
ELISA1/10000
WB (Western Blot)1/500 - 1/2000
Sequence
N
Catalog#: 30057
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Images
Western Blot
Figure 1: Western blot analysis using IHOG mAb against IHOG-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using IHOG mAb against IHOG-hIgGFc transfected HEK293 cell lysate.
Product Overview
Description

The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.

References (references)
References (references)1. Cell. 2006 Apr 21;125(2):343-57.
2. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17208-13.