Mouse Monoclonal Antibody to IGF1R
Item Information
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Size
Price
Description
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Product Overview
Entrez GenelD
3480
Aliases
IGFR; CD221; IGFIR; JTK13
Clone#
3B4E3
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human IGF1R (AA: extra(741-935)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Cells. 2019 Dec 14;8(12):1638.2,Nature. 2020 Jul;583(7817):615-619.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using IGF1R mAb against human IGF1R (AA: extra(741-935)) recombinant protein. (Expected MW is 25.2 kDa)
Western Blot
Figure 3:Western blot analysis using IGF1R mAb against HEK293-6e (1) and IGF1R (AA: extra(741-935))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using IGF1R mouse mAb against NIH3T3 (1), cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using IGF1R mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Lovo cells using IGF1R mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.