IDH2 Primary Antibody

Item Information
Catalog #
Size
Price
Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Product Overview
Entrez GenelD
3418
Aliases
IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
Clone#
3E8E9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human IDH2 (AA: 1-143) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Cancer Res Clin Oncol. 2018 Jun;144(6):1037-1047.
2.J Exp Clin Cancer Res. 2014 Apr 10;33:32.
Product Image
Western Blot
Figure 1:Western blot analysis using IDH2 mAb against human IDH2 (AA: 1-143) recombinant protein. (Expected MW is 42.2 kDa)
Figure 1:Western blot analysis using IDH2 mAb against human IDH2 (AA: 1-143) recombinant protein. (Expected MW is 42.2 kDa)
Western Blot
Figure 2:Western blot analysis using IDH2 mAb against HEK293 (1) and IDH2 (AA: 1-143)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2:Western blot analysis using IDH2 mAb against HEK293 (1) and IDH2 (AA: 1-143)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3:Flow cytometric analysis of Hela cells using IDH2 mouse mAb (green) and negative control (red).
Figure 3:Flow cytometric analysis of Hela cells using IDH2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using IDH2 mouse mAb with DAB staining.
Figure 4:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using IDH2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IDH2 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IDH2 mouse mAb with DAB staining.
Elisa
Figure 6:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 6:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
For Research Use Only. Not for use in diagnostic procedures.