HTRA2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Product Overview

Entrez GenelD

27429

Aliases

OMI; MGCA8; PARK13; PRSS25

Clone#

6H7A8

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Rat

Immunogen

Purified recombinant fragment of human HTRA2 (AA: 278-458) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Br J Cancer. 2015 Feb 17;112(4):739-44.
2.Pathol Res Pract. 2014 Dec;210(12):939-43.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using HTRA2 mAb against human HTRA2 (AA: 278-458) recombinant protein. (Expected MW is 46 kDa)

Western Blot

Figure 3:Western blot analysis using HTRA2 mAb against HEK293 (1) and HTRA2 (AA: 278-458)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using HTRA2 mouse mAb against HL-60 (1), HepG2 (2), MCF-7 (3), Hela (4), PC-12 (5), C2C12 (6), and Raji (7) cell lysate.

Flow cytometric