HTRA2 Primary Antibody
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Description
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HTRA2 mAb against human HTRA2 (AA: 278-458) recombinant protein. (Expected MW is 46 kDa)
Western Blot
Figure 3:Western blot analysis using HTRA2 mAb against HEK293 (1) and HTRA2 (AA: 278-458)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using HTRA2 mouse mAb against HL-60 (1), HepG2 (2), MCF-7 (3), Hela (4), PC-12 (5), C2C12 (6), and Raji (7) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using HTRA2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using HTRA2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using HTRA2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.