HTR3B Primary Antibody

Item Information
Catalog #
Size
Price
Description
The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit B of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It is not functional as a homomeric complex, but a pentaheteromeric complex with subunit A (HTR3A) displays the full functional features of this receptor.
Product Overview
Entrez GenelD
9177
Aliases
5-HT3B
Clone#
2H9H5
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human HTR3B (AA: extra 23-150) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Yonsei Med J. 2015 Sep;56(5):1415-20.
2.Psychiatry Res. 2014 Sep 30;219(1):235-7.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HTR3B mAb against human HTR3B (AA: extra 23-150) recombinant protein. (Expected MW is 41.1 kDa)
Figure 2:Western blot analysis using HTR3B mAb against human HTR3B (AA: extra 23-150) recombinant protein. (Expected MW is 41.1 kDa)
Western Blot
Figure 3:Western blot analysis using HTR3B mAb against HEK293 (1) and HTR3B (AA: extra 23-150)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using HTR3B mAb against HEK293 (1) and HTR3B (AA: extra 23-150)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using HTR3B mouse mAb against SK-N-SH (1) and Hela (2) cell lysate.
Figure 4:Western blot analysis using HTR3B mouse mAb against SK-N-SH (1) and Hela (2) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using HTR3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using HTR3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using HTR3B mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Hela cells using HTR3B mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.