Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HSPA9 mAb against human HSPA9 (AA: 480-679) recombinant protein. (Expected MW is 25.2 kDa)
Western Blot
Figure 3:Western blot analysis using HSPA9 mouse mAb against A549 (1), PANC-1 (2), PC-12 (3), C6 (4), CSO-7 (5)and NIH3T3 (6) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using HSPA9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using HSPA9 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of Jurkat cells using HSPA9 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Flow cytometric analysis of HepG2 cells using HSPA9 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using HSPA9 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSPA9 mouse mAb with DAB staining.