HSPA9

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m
Clone#8D2D11
Entrez GenelD3313
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG2a
ImmunogenPurified recombinant fragment of human HSPA9 (AA: 480-679) expressed in mammalian.
MW74KDa
Species ReactivityHuman, Mouse, Monkey, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
480-679
Catalog#: 32299
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HSPA9 mAb against human HSPA9 (AA: 480-679) recombinant protein. (Expected MW is 25.2 kDa)
Figure 2:Western blot analysis using HSPA9 mAb against human HSPA9 (AA: 480-679) recombinant protein. (Expected MW is 25.2 kDa)
Western Blot
Figure 3:Western blot analysis using HSPA9 mouse mAb against A549 (1), PANC-1 (2), PC-12 (3), C6 (4), CSO-7 (5)and NIH3T3 (6) cell lysate.
Figure 3:Western blot analysis using HSPA9 mouse mAb against A549 (1), PANC-1 (2), PC-12 (3), C6 (4), CSO-7 (5)and NIH3T3 (6) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using HSPA9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using HSPA9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using HSPA9 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using HSPA9 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of Jurkat cells using HSPA9 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Jurkat cells using HSPA9 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Flow cytometric analysis of HepG2 cells using HSPA9 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of HepG2 cells using HSPA9 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using HSPA9 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using HSPA9 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSPA9 mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSPA9 mouse mAb with DAB staining.
Product Overview
Description

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.

References (references)
References (references)1,Cancer Lett . 2021 Apr 1;502:25-33.
2,Exp Mol Pathol . 2021 Feb;118:104593.