Mouse Monoclonal Antibody to HSP70
Item Information
Catalog #
Size
Price
Product Overview
Entrez GenelD
3308
Aliases
RY; APG-2; HSPH2; HSPA4 ; hsp70RY; HEL-S-5a; HS24/P52
Clone#
6H4A5C5
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human HSP70 (AA: 642-841) expressed in Mammalian
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,J Biol Chem. 2020 Jun 12;295(24):8302-8324. 2,Mol Biol (Mosk). Jan-Feb 2020;54(1):128-136.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HSP70 mAb against human HSP70 (AA: 642-841) recombinant protein. (Expected MW is 53.3 kDa)
Western Blot
Figure 3:Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2), Hek293 (3), PC-12 (4), A549 (5), Jurkat (6), COS-7 (7), and NIH/3T3 (8) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder Cancer tissues using HSP70 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSP70 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.