HSP60 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. (provided by RefSeq)
Product Overview
Entrez GenelD
3329
Aliases
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; HSPD1
Clone#
3G8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Rat, Mouse, Monkey
Immunogen
Purified recombinant fragment of human HSP60 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Clin Exp Rheumatol. 2008 Nov-Dec;26(6):1107-10.
2. APMIS. 2008 Oct;116(10):888-95.
2. APMIS. 2008 Oct;116(10):888-95.
Product Image
Western Blot
Figure 1: Western blot analysis using HSP60 mouse mAb against T47D (1), Hela (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using HSP60 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and colon cancer tissues (right) using HSP60 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of 3T3-L1 cells using HSP60 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using HSP60 mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.