HSL Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
Product Overview
Entrez GenelD
3991
Aliases
LIPE; LHS
Clone#
4B7B2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human HSL expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/100
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Mol Endocrinol. 2011 Jan 19;46(1):29-36.
2.Gene. 2011 May 15;477(1-2):1-11.
2.Gene. 2011 May 15;477(1-2):1-11.
Product Image
Western Blot
Figure 1: Western blot analysis using HSL mouse mAb against 3T3L1 (1) and HeLa (2) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of HeLa cells using HSL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using HSL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of HeLa cells using HSL mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.