HSF4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described.
Product Overview
Entrez GenelD
3299
Aliases
CTM
Clone#
2E7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human HSF4 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Apr;26(4):325-8.
Am J Hum Genet. 2009 Nov;85(5):628-42.
Am J Hum Genet. 2009 Nov;85(5):628-42.
Product Image
Western Blot
Figure 1: Western blot analysis using HSF4 mAb against human HSF4 (AA: 245-411) recombinant protein. (Expected MW is 42.9 kDa)
Flow cytometric
Figure 2: Flow cytometric analysis of HeLa cells using HSF4 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.